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ABSTRACT: Alcohol-induced injury has become one of the major causes for liver cirrhosis. However, the molecular mechanisms of ethanol-induced injury are not fully understood. To this end, we performed a dynamic plasma membrane proteomic research on rat model. A rat model from hepatitis to liver cirrhosis was developed. Plasma membrane from liver tissue with liver fibrosis stage of 2 and 4 (S2 and S4) was purified by sucrose density gradient centrifugation. Its purification was verified by western blotting. Proteins from plasma membrane were separated by two-dimensional electrophoresis (2DE) and differentially expressed proteins were identified by tandem mass spectrometry. 16 consistent differentially expressed proteins from S2 to S4 were identified by mass spectrometry. The expression of differentially expressed proteins annexin A6 and annexin A3 were verified by western blotting, and annexin A3 was futher verified by immunohistochemistry. Our research suggests a possible mechanism by which ethanol alters protein expression to enhance the liver fibrosis progression. These differentially expressed proteins might be new drug targets for treating alcoholic liver cirrhosis. 相似文献
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Bai S Zeng R Zhou Q Liao W Zhang Y Xu C Han M Pei G Liu L Liu X Yao Y Xu G 《International journal of biological sciences》2012,8(6):859-869
Cdc42-interacting protein-4 (CIP4) is an F-BAR (Fer/CIP4 and Bin, amphiphysin, Rvs) family member that regulates membrane deformation and endocytosis, playing a key role in extracellular matrix (ECM) deposition and invasion of cancer cells. These processes are analogous to those observed during the initial epithelial-mesenchymal transition (EMT) of renal tubular epithelial cells. The role of CIP4 in renal tubular EMT and renal tubulointerstitial fibrosis was investigated over the course of the current study, demonstrating that the expression of CIP4 increased in the tubular epithelia of 5/6-nephrectomized rats and TGF-β1 treated HK-2 cells. Endogenous CIP4 evidenced punctate localization throughout the cytosol, with elevated levels observed in the perinuclear region of HK-2 cells. Subsequent to TGF-β1 treatment, CIP4 expression increased, forming clusters at the cell periphery that gradually redistributed into the cytoplasm. Simultaneously, EMT induction in cells was confirmed by the prevalence of morphological changes, loss of E-cadherin, increase in α-SMA expression, and secretion of fibronectin. Overexpression of CIP4 promoted characteristics similar to those commonly observed in EMT, and small interfering RNA (siRNA) molecules capable of CIP4 knockdown were used to demonstrate reversed EMT. Cumulatively, results of the current study suggest that CIP4 promotes TGF-β1-induced EMT in tubular epithelial cells. Through this mechanism, CIP4 is capable of inducing ECM deposition and exacerbating progressive fibrosis in chronic renal failure. 相似文献
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马尾松幼苗生长及生理特性对铝胁迫的响应 总被引:4,自引:0,他引:4
以马尾松幼苗为试验材料,采用水培法研究铝胁迫(Al3+浓度为0、0.2、0.4、0.8、1.6 mmol·L-1)对马尾松幼苗生长及其针叶中叶绿素、渗透调节物质(可溶性糖、可溶性蛋白、脯氨酸)、丙二醛(MDA)和超氧化物歧化酶(SOD)、过氧化物酶(POD)等保护酶活性的影响,为揭示马尾松铝毒害生理机制及提高马尾松的耐铝能力提供理论依据。结果显示:当Al3+处理浓度为0.2 mmol·L-1时对马尾松株高和基径生长的影响较小,但对马尾松根系生长有一定的促进作用;Al3+处理浓度大于0.2 mmol·L-1时对马尾松株高、基径和根长的生长均会产生一定的抑制作用,且这种抑制作用随着Al3+浓度的增大而增强。马尾松针叶中叶绿素含量和SOD、POD活性均随着Al3+处理浓度的增加呈先上升后下降的趋势;Al3+处理浓度大于0.2 mmol·L-1时马尾松针叶中可溶性糖、可溶性蛋白、脯氨酸等渗透调节物质均呈上升趋势,且随着Al3+浓度的升高而增大;Al3+处理浓度大于0.2 mmol·L-1时马尾松针叶中MDA含量也呈上升趋势,且随着Al3+浓度的增大而升高,说明大于0.2 mmol·L-1的Al3+处理可导致马尾松膜脂产生氧化。研究表明,马尾松幼苗具有一定的耐铝能力,在铝胁迫生境下可通过提高自身SOD和POD等保护酶的合成和主动积累脯氨酸、蛋白质和可溶性糖等渗透物质,产生适应性生理响应以维持自身的生理平衡来降低铝毒害作用。 相似文献
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Fisetin provides antidepressant effects by activating the tropomyosin receptor kinase B signal pathway in mice 下载免费PDF全文
Yamin Wang Bin Wang Jiaqi Lu Haixia Shi Siyi Gong Yufan Wang Ronald C. Hamdy Balvin H. L. Chua Lingli Yang Xingshun Xu 《Journal of neurochemistry》2017,143(5):561-568
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Arsenic contamination in groundwater has been reported in the Jianghan Plain of China since 2005, yet little is known about the microbial communities involved in As mobilization in this area, especially the dissimilatory arsenate-reducing bacteria (DARB) communities. Here, we conducted a cultivation-independent investigation on core sediments collected from a region with arsenic-contaminated groundwater in the Jianghan Plain to reveal the total bacteria and DARB community structures. Highly diverse As-resistant bacteria communities were found from sediment samples via high-throughput sequencing of 16S rRNA genes. Notably, we identified 27 unique arrA gene (encoding the alpha subunit of dissimilatory arsenate reductase) phylotypes, none of which was related to any previously described arrA gene sequence. This suggests a novel and unique DARB community in the sediments of the Jianghan Plain and expands our knowledge about the distribution and diversity of this group of bacteria in natural environments. Moreover, RDA and CCA demonstrated that total bacterial communities and specific functional groups are controlled by different environmental factors. Specifically, sediment pH, NH4+, total nitrogen, total Fe, total organic carbon and total phosphorus were the key factors driving total bacterial community compositions, while As significantly shaped DARB community structures. This report is the first to describe DARB communities and their correlation with environmental factors in Jianghan Plain sediments, which could give us clues about the origin of the arsenic contamination of groundwater in this region. 相似文献
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Weiqiong Gan Li Deng Chen Yang Qingfeng He Huan Yin Xian Jin Yamin Wu 《FEBS letters》2009,583(12):1976-1980
A full-length cDNA encoding an anticoagulant peptide, named AduNAP4, was cloned and identified from the human hookworm Ancylostoma duodenale. AduNAP4 has 104 amino acids including a predicted 23-residue signal peptide and shows ?50% similarity with other known nematode anticoagulant protein/peptide (NAP). AduNAP4 is extremely efficient at prolonging the activated partial thromboplastin time, and is an inhibitor of both fXa (Ki = 7.34 ± 1.74 nM) and fXIa (Ki = 42.45 ± 3.25 nM). No fXIa inhibitor has previously been described from other blood-feeding animals. Our results suggest that hookworms have evolved a potent mechanism that interferes with coagulation by inhibition of fXIa to facilitate its blood-feeding lifestyle. 相似文献
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Lowe DB Bifulco N Bullock WH Claus T Coish P Dai M Dela Cruz FE Dickson D Fan D Hoover-Litty H Li T Ma X Mannelly G Monahan MK Muegge I O'Connor S Rodriguez M Shelekhin T Stolle A Sweet L Wang M Wang Y Zhang C Zhang HJ Zhang M Zhao K Zhao Q Zhu J Zhu L Tsutsumi M 《Bioorganic & medicinal chemistry letters》2006,16(2):297-301
A series of oxazole-substituted indanylacetic acids were prepared which show a spectrum of activity as ligands for PPAR nuclear receptor subtypes. 相似文献